Calcium-binding properties of human erythrocyte calpain

Calcium binding capacity of erythrocyte membrane in human hypertension.

The cell membrane calcium binding capacity of genetically hypertensive rats is reduced when measured in the presence of the submicromolar calcium concentrations proper of intracellular environment. The present work, performed as an ancillary study to an epidemiological survey on an entire population, aimed to investigate the existence of a similar abnormality in human hypertension. Calcium bind...

Properties of erythrocyte membrane binding and autolytic activation of calcium-activated neutral protease.

The binding of a calcium-activated neutral protease (CANP) with high calcium sensitivity (muCANP) to erythrocyte membranes and its subsequent autolytic activation on the membranes were analyzed by an immunoblot technique. In the presence of calcium ions, muCANP bound to the erythrocyte membranes as a heterodimer of 79- and 28-kDa subunits and was converted quickly on the membranes to an active ...

Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during Pregnancy

Erythrocyte membrane polyphosphoinositide metabolism and the regulation of calcium binding.

The terminal phosphate of ATP is incorporated into erythrocyte membrane polyphosphoinositides at the rate of 1.5 nmoles per min per mg of protein in the presence of 10 mM Mg2+ and 0.5 mu ATP. The amount of membrane polyphosphoinositide monophosphate can be doubled in 30 min. Phosphorylation proceeds until the entire supply of membrane phosphatidylinositol is exhausted. Inhibition of incorporati...

Calcium and Calpain Activation

Even with clinical treatment to reduce intraocular pressure (IOP), visual field defects still progress in many patients. Recent research has shown that the optic nerve head (ONH) is a key location in this process, where mechanical, ischemic, and inflammatory stresses are associated with axonal transport defects and retinal ganglion cell (RGC) apoptosis. Glutamate, an excitatory neurotransmitter...